Human COX1, GenBank Accession No. NM_000962, a.a. 1-599 (end), with C-terminal FLAG-His-tag, MW=71 kDa, expressed in a baculovirus infected Sf9 cell expression system.

P23219

Cox, COX1, COX 1, COX 2, COX2, COX1 Protein, Cyclooxygenase-1, Cox-1, Prostaglandin-Endo-peroxide Synthase 1, Prostaglandin G/H Synthase and Cyclooxygenase, PTGS1, PES-1, PGHS-1, Prostaglandin H2 Synthase 1, PTGHS, COX1

≥90%

Mixed various concentrations (0-1410 ng) of enzyme in Assay buffer (100 mM Tris-HCl pH 8, 3 µM EDTA) with 5 uM Hematin for 5 min then added 100 µM Ampliflu Red and 50 µM arachidonic acid (Cayman Chemical # 90010.1 peroxide free) and measured fluorescence (ex 535 em 590) after 5 min. Reaction volume was 100 µl.

40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 0.04% Tween20, 80µg/ml FLAG peptide, 300µM diethyldithiocarbamate (DDC), 0.08% Triton X-100 and 20% glycerol.

Aqueous buffer solution.

At least 6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Scott, B.T., et al., Blood Coagul Fibrinolysis. 2002 Sep; 13(6):519-31.

2. Funk, C.D., et al., FASEB J. 1991 Jun; 5(9):2304-12.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Metabolic Enzymes

Protein