Human carbonic anhydrase II, also known as CA2, (GenBank Accession No. NM_000067), full length (a.a. 1-260(end)) with C-terminal His-tag, MW = 30 kDa, expressed in an E. coli cell expression system.

P00918

carbonic anhydrase II, CA2

1,400 pmol/min/μg

15 mM Tris-HCl, pH 7.4, 100 mM NaCl, 3 mM p-Nitrophenyl acetate (PNPA) and 0-2 μg CA2. Incubate for 30 minutes at room temperature. Absorbance is measured at 405 nm.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 106 mM imidazole and 20% glycerol

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Loiselle, F.B., et al., Am J Physiol Cell Physiol. 2004 Jun;286(6):C1423-33
2. Shah, G.N., et al., Hum Mutat. 2004 Sep;24(3):272

Avoid freeze/thaw cycles.

Metabolic Enzymes-Anhydrase

Recombinant Protein