| 产品名称 | IDO1 Cell Line |
| 产品货号 | 60532 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 2 vials |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Genbank #: NM_002164
Background: L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in
mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key
regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an
immunosuppressive microenvironment by starving immune cells of L-Trp and releasing
degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3-
dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated
in many tumors, providing cancer cells with an avenue for immune evasion.
Description: Stable Recombinant HEK293 IDO1 cell line expressing tetracycline-inducible human indoleamine 2,3-dioxygenase (IDO1) Genbank accession number NM_002164. Materials Required But Not Provided: • Thaw Medium 1 (BPS Cat. #60187) Synonym(s): Indoleamine 2,3-dioxygenase 1, IDO
Supplied As: Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO
Mycoplasma Testing: The IDO1 cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit
(Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Growth Media: Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01). Instructions for use: See data sheet for detailed culturing and assay protocol
Storage / Stability: Immediately upon receipt, store vials in liquid nitrogen.
Application(s): Monitor IDO1 pathway activity. Screen for activators or inhibitors of IDO1 in a cellular context
Reference(s): 1. Yue, E., et al., J. Med. Chem. 2009; 52: 7364–7367. Notes: License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Immunotherapy
Product Type: Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.
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