| 产品名称 | TDO Fluorogenic Inhibitor Screening Assay Kit (Human) - Assay Kits - Metabolic Enzymes |
| 产品货号 | 72039 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 96 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: L-tryptophan (L-Trp) is an essential amino acid necessary for protein
synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as
a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an
immunosuppressive microenvironment by starving immune cells of L-Trp and releasing
degradation products of L-Trp that have immunosuppressive functions., Tryptophan 2,3
dioxygenase (TDO) a rate limiting enzymes in this pathway, is upregulated in many tumors,
providing cancer cells with an avenue for immune evasion.
Description: The TDO Fluorogenic Inhibitor Screening Assay Kit is designed to measure enzyme inhibition of tryptophan 2,3-dioxygenase (TDO). The TDO Fluorogenic Inhibitor Screening Assay Kit is simple to use and detects fluorescence at long wavelengths, which minimizes potential errors due to compound interference. In the assay, the inhibitor and enzyme are added to a sample containing L-Trp substrate. After a 1 hour incubation at room temperature, the fluorescence solution is added and incubated at 37°C for four hours. Activity is measured by reading sample fluorescence at λ=510 nm following excitation of the reaction product at l=400 nm. Synonym(s): TDO2, tryptophan 2,3 dioxygenase, activity assay kit
Supplied As: The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 96 reactions.
Contraindications: DMSO >0.5%, strong acids or bases, ionic detergents, high salt.
Warning: the Fluorescence Solution contains a component that is known to be a skin and eye
irritant, use caution and appropriate personal protective equipment when handling this
component.
Format: COMPONENTS:
Instructions for use: See assay kit data sheet for detailed protocol
Storage / Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s): Useful for the study of TDO enzymology, inhibitor screening, and selectivity profiling.
Reference(s): Seegers, N., et al. J. Biomol. Screen. 2014. 19(9):1266-74. Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Immunotherapy
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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