Acetyl-Coenzyme A Carboxylase (ACC) plays an important role in fatty acid metabolism so it has been proposed to be a drug target for the fatty acid-related metabolic diseases including obesity and diabetes. The Acetyl-Coenzyme A Carboxylase 1 (Human ACC1) assay kit is designed to measure ACC1 activity for screening and profiling applications using ADP-Glo™ reagents as a detection reagent

acetyl-Coenzyme A carboxylase alpha transcript variant 1, ACC-1, ACC 1, ACC -1, acetyl-coa carboxylase, acetyl coa , acetyl coenzyme a

The Acetyl-coenzyme A carboxylase 1 (ACC1) assay kit comes in a convenient 96-well format, with enough purified recombinant ACC1 enzyme, ACC1 substrate, ATP and ACC assay buffer for 100 enzyme reactions.

Catalog #	Reagent	Amount	Storage
50202	ACC1 (Human)	10 µg	-80°C	Avoid multiple freeze/ thaw cycles!
79283	5x ACC assay buffer	1 ml	-20°C
	ATP (500 µM)	100 µl	-20°C
	Acetyl-CoA (2 mM)	25 µl	-20°C
	Sodium Bicarbonate (1 M)	75 µl	-20°C.
	96-well plate, white	1	Room Temp.

See assay kit data sheet for detailed protocol.

Stable up to 6 months when stored as recommended. Kit components require different storage conditions, Be sure to store each component at the proper temperature upon arrival.

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Ting, L. J. Cell. Biochem.99(6):1476-1488 (2006).

Avoid freeze/thaw cycles.

Metabolic Enzymes

Assay Kit