| 产品名称 | ATP Citrate Lyase Protein |
| 产品货号 | 50255 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 147 kDa
Genbank #: NM_001096
Tag(s): N-terminal GST-tag
a.a: full length
Background: ATP citrate-lyase is an important enzyme for cytosolic acetyl-CoA synthesis. During fatty acid synthesis, Citrate and CoA form acetyl-CoA and oxaloacetate. ATP citrate-lyase has emerged as new target for cancer treatment. Studies have found that many types of cancer including glioblastoma, colorectal cancer, breast cancer, non-small cell lung cancer, and hepatocellular carcinoma experience abnormally high levels of ATP citrate-lyase. Description: Human ATP citrate lyase (ACLY) (GenBank Accession No. NM_001096), full length, with N-terminal GST-tag, MW=147 kDa, expressed in baculovirus expression system.
UniProt P53396
Synonym(s): ATP citrate lyase, ACLY, ACL
Specific Activity: ≥300 pmole/min/μg.
Assay Conditions: A 25 µl ACLY reaction is conducted in a buffer
containing 40 mM Tris (pH 8.0), 10 mM MgCl2,
2 mM DTT, 2 mM potassium citrate, 0.1 mM CoA
and 6-100 ng human ACLY at 30 oC for 60 min.
Enzyme activity was assayed using ADP-Glo
reagents (Promega)
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, and 3 mM DTT
Format: Aqueous buffer solution
Storage / Stability: >6 months at –80°C Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Migita T. et al., Cancer Res. 68 (20), 8547- 8554 (2008). Application Reference:
Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Metabolic Enzymes-Lyase
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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