Recombinant human soluble BAFF (GenBank Accession No. NM_006573) is a 17.0 kDa (a.a. 134-285) protein expressed in an E. coli expression system.

Q9Y275

BAFF, BLYS, TALL-1, CD257, TNFRSF13C, B-cell activating factor, TNF13b

≥98% by SDS-PAGE and HPLC analysis.

< 1EU per µg of BAFF as determined by the LAL method.

Measured by its ability to stimulate human B lymphocyte cells. The ED50 of this effect is between 5 - 10 ng/ml

Lyophilized from a 0.2 µm filtered solution containing 0.5X PBS, pH 7.5 + 0.5 mM DTT, containing no additives.

lyophilized protein

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

≥2 years (lyophilized) at -20°C.

Useful for cell culture and for the study of signaling pathways.

1. Knight AK, et al. Clin. Immunol. 124 (2), 182-189 (2007)
2. Hever, A., et al. Proc. Natl. Acad. Sci. U.S.A. 104 (30), 12451-12456 (2007)

Avoid freeze/thaw cycles.

Cytokine

Recombinant Protein