The CD40:CD40L TR-FRET Assay is designed to measure the inhibition of CD40 binding to CD40L in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated CD40, CD40L, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.

T cell antigen Gp39, TNF related activation protein, TRAP, tumor necrosis factor ligand superfamily member 5, TNFSF5, CD154, CD40, CD40 Molecule, HTRF

This kit comes in a convenient 384-well format.

See assay kit data sheet for detailed protocol

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for screening small molecular inhibitors for drug discovery and HTS applications.

Avoid freeze/thaw cycles.

Immunotherapy

Assay Kit