世联博研公司现货供应bpsbioscience品牌订货号为72008的BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit - Assay kits

产品名称

BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit - Assay kits - Immunotherapy

产品货号

72008

产品价格

现货询价，电话：010-67529703

产品规格

96 reactions

产品品牌

bpsbioscience

产品概述

产品详情

Description:

The binding of BTLA to the HVEM receptor (herpesvirus entry mediator, also known as TNFRSF14) triggers cell signaling pathways involved in the negative regulation of T cells. BTLA:HVEM interaction plays a key role in the regulation of inflammatory, autoimmune, and antitumor responses, and is an important target for drug discovery. The BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of BTLA:HVEM signaling. The key to this kit is the high sensitivity of detection of biotin-labeled HVEM by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, BTLA is coated on a 96-well plate. Next, HVEM is incubated with BTLA on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Materials Required But Not Provided:

PBS (Phosphate buffered saline)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Rotating or rocker platform

Synonym(s):

CD272, CD270, TNFRSF14, ATAR, HVEA, LIGHTR, TR2 , activity assay kit, Strep HRP, streptavidin

Supplied As:

This kit comes in a convenient 96-well format, with biotin-labeled HVEM, purified BTLA, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Format:

Catalog #	Component	Amount	Storage
71143	HVEM, Biotin-labeled	5 µg	-80°C	*(Avoid freeze/ thaw cycles!)*
71141	BTLA	10 µg	-80°C
	Streptavidin-HRP	15 µl	+4°C
79311	3x Immuno Buffer	50 ml	-20°C
79728	Blocking buffer 2	50 ml	+4°C
	HRP chemiluminescent substrate A (transparent bottle)	6 ml	+4°C
	HRP chemiluminescent substrate B (brown bottle)	6 ml	+4°C
79699	White 96-well microplate	1	+4°C

Instructions for use:

See assay kit data sheet for detailed protocol

Storage / Stability:

One year from date of receipt when stored as directed.

Application(s):

This kit is useful for screening inhibitors of HVEM binding to BTLA

Reference(s):

1. Derre L., et al. J. Clin. Invest. 2010, 120(1): 157-167.
2. Steinberg, M.W., et al. J. Exp.Med. 2008, 205(6): 1463-1476.

Warning(s):

Avoid freeze/thaw cycles.

Scientific Category:

Cell Surface Receptor/Single-pass Transmembrane Protein

Product Type:

Assay Kit

Data shown is lot-specific. Contact us for specific information on other lots.

Qty:

Quality Assurance

Product Datasheets

72008.pdf

产品资料