• Thaw Medium 3 (BPS Bioscience, #60186)
• Growth Medium 3D (BPS Bioscience, #79539)
• 96-well tissue culture-treated white clear-bottom assay plate
• ONE-Step luciferase assay system (BPS Bioscience, #60690) or other luciferase reagent for measuring firefly luciferase activity
• Luminometer

Thaw Medium 3 (BPS Bioscience, #60186): Ham’s F-12 medium (Hyclone, #SH30526.01) supplemented with 10% FBS (Life technologies, #26140-079) and 1% Penicillin/Streptomycin (Hyclone, #SV30010.01).

Growth Medium 3D (BPS Bioscience, #79539): Thaw medium 3 (BPS Bioscience, #60186) plus 1 mg/ml of Geneticin (Life technologies, #11811031) to ensure recombinant expression.

License Disclosure
Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate
laboratory, for research use only. This license does not permit you to share, distribute, sell,
sublicense, or otherwise make the cell line available for use to other laboratories, departments,
research institutions, hospitals, universities, or biotech companies. The license does not permit
use of this cell line in humans or for therapeutic or drug use. The license does not permit
modification of the cell line in any way. Inappropriate use or distribution of this cell line will result
in revocation of the license and result in an immediate cease of sales and distribution of BPS
products to your laboratory. BPS does not warrant the suitability of the cell line for any particular
use, and does not accept any liability in connection with the handling or use of the cell line.
Modifications of this cell line, transfer to another facility, or commercial use of the cells may
require a separate license and additional fees; contact sales@bpsbioscience.com for
details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.