Biotin ligase BirA, also known as Holocarboxylase Synthetase, Biotin-Protein Ligase, and ATP-Hydrolysing Ligase, GenBank Accession No. NP_418404, a.a. 2–321 (end) with N-terminal His-FLAG tag, expressed in an E. coli cell expression system. MW = 37 kDa.
| 产品名称 | BirA, His-FLAG-tag (E. coli-derived) - BirA Proteins - Biotinylation |
| 产品货号 | 70030 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 100 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 37 kDa
Genbank #: NP_418404
Tag(s): N-terminal His-FLAG-tag
a.a: 2–321
Description: UniProt H0QFJ5
Synonym(s): Holocarboxylase Synthetase, Biotin Protein Ligase, ATP-Hydrolysing Ligase,
Assay Conditions: BirA (10 µg/ml)
completely biotinylated 10 µM of
substrate MBP-Avi at 30oC for 30
minutes in the standard reaction buffer
containing 50 mM Bicine pH 8.3, 10 mM
ATP, 10 mM MgOAc, and 50 µM d-Biotin.
Formulation: 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 150 mM imidazole, 3 mM DTT, and 5% glycerol.
Format: Aqueous buffer solution
Storage / Stability: At least 6 months at –80°C.
Application(s): Useful for covalently biotin-labeling of acceptor proteins for detection and purification assays.
Reference(s): 1. Li, Y., et al., Protein Expr Purif. 2012 Mar; 82(1): 162-7.
2. Chakravartty, V., et al., J Bacteriol. 2012 Mar; 194(5): 1113-26. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Miscellaneous
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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