| 产品名称 | E. coli in vivo Biotinylation Kit - Biotinylation Assay Kits - Biotinylation |
| 产品货号 | 27461 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 x 1L* |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: Biotin-labeling is commonly used for non-radioactive labeling and purification of proteins and other target molecules. Biotin labeling takes advantage of the exceptionally strong interaction between biotin (vitamin H) and either avidin or streptavidin. The affinity of biotin to avidin or streptavidin is one of the strongest known non-covalent interactions of a protein and ligand, exhibiting a dissociation constant (Kd) around 10-15. BirA protein ligase covalently adds biotin to biotin-acceptor peptides containing an AviTag™ sequence in a highly efficient and specific manner in a reaction that requires ATP.
Description: The E. coli in vivo biotinylation kit comes in a convenient, easy-to-use format and contains all the components necessary to label biotin-acceptor peptides with biotin in E. coli. Strain BL21, a chemically competent E. coli B strain. The cells include an IPTG-inducible plasmid containing the BirA gene and the resistance gene for streptomycin or spectinomycin. Just transform the competent cells with a vector expressing your protein of interest with an AviTag™ sequence (example: pAN or pAC vectors). After transformation, BirA expression is induced and biotin is added to the culture. The BirA efficiently catalyzes the specific attachment of biotin to the AviTag.
Format: Components:
Storage / Stability: One year from date of receipt when stored as directed.
Application(s): Useful for specific biotin labeling of proteins/peptides containing the AviTag sequence for downstream applications.
Reference(s): Fairhead, M, and Howarth, M. Methods Mol Biol. 2015; 1266: 171–184.
Notes: *This kit contains sufficient reagents to label 10 x 1 L of E. coli culture
Scientific Category: Biotinylation
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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