Human NLR Family Pyrin Domain Containing 3 (NLRP3), also known as NALP3, Cold Autoinflammatory Syndrome 1 Protein (CIAS1), cryopyrin and PYPAF1 (Genbank Accession No. NM_004895), a.a. 2-1036(end) with N-terminal His-FLAG-tag, expressed in a baculovirus infected Sf9 cell expression system. MW = 120 kDa (calculated).

Q96P20

NLRP3, CIAS1, cryopyrin, PYPAF1, NLR Family Pyrin Domain Containing 3, Cold Autoinflammatory Syndrome 1 Protein, NALP3

≥50%

aqueous buffer solution

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol

Aqueous buffer solution

>6 months at –80°C

Useful for protein binding studies, screening inhibitors of protein-protein interactions, and studying protein function.

1. Kummer, J.A., J. Histochem. Cytochem., 2007 May; 55(5):443-52.
2. Manji, G.A., J. Biol. Chem. 2002 Mar 29; 277(13):11570-5.

Avoid freeze/thaw cycles

MHHHHHHDYKDDDDKKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLP
RGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDN
ARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNA
RLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDE
HSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSL
GDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDIL
LSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEA
QARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLS
SLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLR
MNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLL
ENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKL
QIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESL
SLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSV
LSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVL
SSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVL
STSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVL
STNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLT
SSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQ
EEKPELTVVFEPSW

Apoptosis

Recombinant Protein