The BCL2L2 TR-FRET Assay Kit is designed to measure the inhibition of BCL2L2 (BCL-w) binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BCL2L2, peptide ligand, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.

BCL2L2, BCL-W, Bcl-2-like protein 2, BCL2 like 2, HTRF

The BCL2L2 TR-FRET Assay Kit is designed to measure the inhibition of BCL2L2 (BCL-w) binding to its ligand in a homogeneous 384 reaction format.

See assay kit data sheet for detailed protocol.

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for screening small molecular inhibitors for drug discovery and HTS applications.

Kvansakul, M., Hinds, MG. Cell Death and Disease 2013; 4: e909

Avoid freeze/thaw cycles.

Apoptosis

Assay Kit