Mouse secreted programmed cell death 1 (PD-1)-Fc fusion protein, also known as, PDCD1, SLEB2, CD279 and HPD-L, GenBank Accession No. NM_008798, a.a 25-167, fused at the C-terminus to the Fc portion of Human IgG1, expressed in a HEK293 cell expression system. MW = 43 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation. The protein was randomly labeled with RPE on accessible lysine residues.

Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L

8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol.

At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 μg/ml in PBS + glycerol and stored at -80°C.

Useful for labeling PD-L1 expressing cells for flow cytometry or immunohistochemistry.

1. Agata, A., et al., Immunol. 1996 Oct;8(5):765-772.

2. Freeman, G., et al., JEM. 2000 Oct; 192(7):1027-1034.