| 产品名称 | LSD1 (KDM1A) Homogeneous Assay Kit |
| 产品货号 | 50108 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 384 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development.
Description: The LSD1 Homogeneous Assay Kit is designed to measure activity of
lysine-specific demethylase (LSD1) for screening and profiling applications. The key to the LSD1 Homogeneous
Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this
kit, only three simple steps on a microtiter plate are required for demethylase detection.
First, a sample containing LSD1 enzyme is incubated with the biotinylated substrate. Next,
acceptor beads and primary antibody are added, then donor beads, followed by reading
the Alpha-counts.
UniProt O60341
Synonym(s): KDM1A, AOF2, LSD1, BHC110, LSD, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen
Format:
Instructions for use: See assay kit data sheet for detailed protocol.
Storage / Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. Application(s): Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s): 1. Forneris F, et al. J Biol Chem 2006; 281(46):35289-95.
2. Zhou M, et al. Anal Biochem 1997; 253(2):162-8. Notes: AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Warning(s): Avoid freeze/thaw cycles
Scientific Category: Demethylase
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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