Human HDAC11 (full length) (GenBank Accession No. NM_024827) untagged, MW= 39.6 kDa, expressed in baculovirus expression system.

Q96DB2

histone deacetylase 11, HDAC-11

≥ 1500 pmol/min/µg

25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, 0.05% Triton X-100, 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 1 (Catalog #50040), and HDAC11 (0.4 – 25 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature.

50 mM Tris-HCl, pH 8.0, 500 mM NaCl, 50 mM KCl, 2 mM EDTA, 3 mM DTT, 0.5% Triton-X-100 and 10% Glycerol.

Aqueous buffer solution

>6 months at -80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002).

Application Reference:
Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014)

Avoid freeze/thaw cycles.

Deacetylase

Recombinant Protein