Human VEGFR2 (GenBank Accession No. AF035121), a.a. 805-1356 with N-terminal GST-tag, MW=87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

KDR, Kinase Insert Domain Receptor, VEGFR2, Vascular Endothelial Growth Factor Receptor 2, CD309, FLK-1, Fetal Liver Kinase 1

≥400 pmol/min/μg

One unit of KDR activity is defined as the amount of enzyme required to produce 1 pmol of phosphorylated Tyr peptide 1 per min at 37°C.

Assay was done in Kinase buffer containing 0.2 mM DTT using Poly- (Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween, 20% glycerol, 3 mM DTT

Aqueous buffer solution

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Meyer, M. et al., EMBO J. 18, 363-374 (1999).
2. Rahimi, N. et al., J. Biol Chem 275, 16986- 16992 (2000).

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein