| 产品名称 | PCSK9-LDLR TR-FRET Assay Kit | ||||||||||||||||||||||||||
| 产品货号 | 72010 | ||||||||||||||||||||||||||
| 产品价格 | 现货询价，电话：010-67529703 | ||||||||||||||||||||||||||
| 产品规格 | 384 reactions | ||||||||||||||||||||||||||
| 产品品牌 | bpsbioscience | ||||||||||||||||||||||||||
| 产品概述 | |||||||||||||||||||||||||||
| 产品详情 |
Background: PCSK9 is a crucial player in the regulation of plasma cholesterol homeostasis. It binds to the ectodomain of hepatic low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation. PCSK9 acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation
Description: The PCSK9/LDLR TR-FRET Assay Kit is designed to measure the inhibition of PCSK9
binding to LDLR in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing
steps, making it especially suitable for high throughput screening applications. The
assay procedure is straightforward and simple; a sample containing europium-labeled
(Eu) LDLR ectodomain, dye-labeled acceptor, biotin-labeled PCSK9, and an inhibitor is
incubated for two hours. Then, the fluorescence intensity is measured using a
fluorescence reader.
UniProt Q8NBP7
Synonym(s): pcsk9, cholesterol, LDL, kexin, HTRF
Supplied As: Kit comes in a convenient format with biotinylated PCSK9, Eu-labeled LDLR, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.
Format:
Instructions for use: See assay kit data sheet for detailed protocol.
Storage / Stability: At least 6 months from date of receipt when stored as directed.
Application(s): Great for screening small molecular inhibitors for drug discovery and HTS applications.
Notes: MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Adjustable micropipettor and sterile tips Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Cholesterol Related
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
|
||||||||||||||||||||||||||
| 产品资料 |
