Human MAT2A, also known as methionine adenosyltransferase II alpha, MAT2, and adoMet synthase 2. GenBank Accession No. NM_005911, a.a. 2-395 (end) with an N-terminal His-tag, expressed in an E. coli cell expression system. MW = 45 kDa.

MATA2; MATII; SAMS2; S-adenosylmethionine synthase isoform type-2; methionine adenosyltransferase II, alpha; MAT 2; MAT-II; adoMet synthetase 2; methionine adenosyltransferase 2; adoMet synthase 2; testicular tissue protein Li 121

The reactions were conducted at room temperature for 45 minutes in a 25 µl mixture containing 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM KCl, 0.1 mg/ml BSA, various amount of MAT2a enzyme, 200 μM L-Methionine, and 200 μM ATP. After the enzymatic reactions, 50 μl of colorimetric detection reagent was added to each well and the reactions were incubated for another 15 min before the absorbance at 630 nm of each well was measured using a Tecan Infinite M1000 microplate reader.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween, 20% glycerol, additional 250mM imidazole.

Aqueous buffer solution

At least 6 months at –80°C.

Useful for studying protein binding and screening small molecules.

1. Latasa, M., et al., The FASEB Journal. 2001; 15(7): 1248-1250.

2. Liu, Q., et al., Molecular Cancer Therapeutics. 2011; 10(6):1113-23

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C. 

Metabolic Enzymes

Protein