Human phosphoglycerate dehydrogenase (PGDH), also known as PDG, a.a. 1 – 533 (end) (GenBank Accession No. NM_006623) with C-terminal His-tag, MW = 57.5 kDa, expressed in an E. coli expression system.

O43175

phosphoglycerate dehydrogenase, PGDH, PDG

≥80 pmol/min/µg

The reaction mixture contained 100 mM Tris-HCl, pH 8.8, 400 mM NaCl, 0.2 mM DTT, 2.5 mM 3-phospho-D-glyceric acid (Sigma P8877), 1 mM NAD, and various amount of PHGDH in a final volume of 50 µL. Reactions were initiated with the addition of PHGDH, and the increase in NADH fluorescence (Ex340/Em460 nm) was continuously monitored for 10 min at room temperature.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 170 mM imidazole, 0.04% Tween-20, and 20% glycerol.

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Mullarky, E., et al., Pigment Cell Melanoma Res. 2011 Dec;24(6):1112-5.
2. Liu, J., et al., J Neurooncol. 2013 Feb;111(3):245-55.

Avoid freeze/thaw cycles.

Metabolic Enzymes-Dehydrogenase

Recombinant Protein