Complex of recombinant, full length human CDK4 (GenBank accession number NM_000075) with C-terminal His-tag and modifications (a.a. 43G-47G were replaced with EE, and T172A), and human Cyclin D1 (GenBank accession number NM_053056), a.a. 16-271, were co-expressed in a baculovirus-infected Sf9 cells expression system. CDK4 MW=34 kDa, Cyclin D1 MW=29 kDa.

P11802 (CDK4), P24385 (cyclin D1)

Cyclin-Dependent Kinase 4, Cell Division Kinase 4, CDK4, Cyclin D1, CCD1, Bcl1, PRAD1, CCND1

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at -80°C.

Useful for three dimensional structure studies and binding assays.

1. Amendola D. et al., Cell Prolif. 2009. Feb; 42(1):94-109.
2. Yang L. et al., Biochem Cell Biol. 2009. Dec; 87(6):933-42.

Application Reference(s):
1. Iterative conversion of cyclin binding groove peptides into druglike CDK inhibitors with antitumor activity (2015)

Avoid freeze/thaw cycles

Kinase/Serine-Threonine

Recombinant Protein