| 产品名称 | AMPK (A1/B1/G1), His-tags |
| 产品货号 | 40025 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: ~146 kDa (complex)
Genbank #: NM_006251(A1), NM_006253(B1), NM_002733 (G1)
Tag(s): C-terminal His-tags
a.a: full length
Background: AMPK is a heterotrimer protein kinase consisting of an α catalytic subunit, and non-catalytic β and γ subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status (1). In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol (2).
Description: Recombinant full-length
human AMPK (combination of A1/B1/G1
subunits) was expressed by baculovirus in
Sf9 insect cells using a C-terminal His tag.
The gene accession number for the three
subunits (A1/B1/G1) is NM_006251,
NM_006253, and NM_002733,
respectively. This protein has been
activated with CAMKK.
Synonym(s): Subunit A1: PRKAA1, MGC33776, MGC57364
Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G1: PRKAG1, AMPKG, MGC8666 Purity: ≥95%
Specific Activity: ≥700 pmole/min/µg
Assay Conditions: Assay was done in Kinase
buffer containing 1 mM DTT using AMARA
peptide as a substrate (0.1 mg/ml), 20 μM
ATP and 100 μM AMP . Reaction was
done at 30°C for 45 min. Amount of ATP
transferred was calculated using Kinase-
Glo reagent (Promega)
Formulation: 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Minokoshi, Y. et al. Nature 428: 569-574, 2004.
2. Hardie, D G. et al. Eur J Biochem. 1997 Jun 1,246(2):259-73. Warning(s): Avoid freeze/thaw cycles. Storing diluted enzyme
is not recommended, if necessary, use
carrier protein (BSA 0.1 – 0.5%).
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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| 产品资料 |
