Human acetyl-Coenzyme A carboxylase alpha transcript variant 2 (ACC2), GenBank Accession No. NM_001093, full length, with C-terminal His-tag, MW=277 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

acetyl-coa carboxylase, acetyl coa, acetyl coenzyme a, ACC2, acetyl-Coenzyme A carboxylase alpha transcript variant 2, ACC-2, ACC 2, ACC -2

≥20 pmoles/min/µg

25 μl ACC2 reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl2, 2 mM potassium citrate, 1 mM DTT, 100 μM ATP, 12 mM NaHCO3 and 20 μM acetyl-CoA at 30°C for 45 min. ATP reduction is detected using ADP-Glo® Luminescent Assay Platform (Promega).

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 20% glycerol and 100 mM Imidazole.

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Locke G.A. et al., Arch. Biochem. Biophys. 475 (1), 72-79 (2008).
2. Abu-Elheiga L. et al., Proc. Natl. Acad. Sci. U.S.A. 97 (4), 1444-1449 (2000).

Application Reference(s):
A Cellular Assay for Inhibitors of the Fatty Acid Biosynthetic Pathway Using Scintillating Microplates (2015)

Avoid freeze/thaw cycles.

Metabolic Enzymes-Carboxylase

Recombinant Protein