The fluorogenic DPP substrate 1 is a Ala-Pro-AMC dipeptide. It can be used for the activity assays of DPP4, DPP7, DPP8, DPP9, FAP, and prolyl oligopeptidase (POP). λex 353-380 nm, λem=440-460 nm

Ala-Pro-AMC, A-P-AMC

0.5 mM solution in DMSO (100 µl)

DMSO solution

Stable at least 6 months from date of receipt, when stored as directed

Useful for the study of DPP activity, and for screening small molecule inhibitors of DPP4, DPP7, DPP8, DPP9, FAP, and POP for drug discovery and HTS applications. Also suitable for use with Xaa-Pro dipeptidase.

1. Park, J.E., et al., J. Biol. Chem., 274, 36505 (1999).
2. Sun, S., et al., Protein Expr. Purif., 24, 274 (2002).

Application Reference(s):
1. Design, synthesis and anti-diabetic activity of triazolotriazine derivatives as dipeptidyl peptidase-4 (DPP-4) inhibitors (2017)

Protect from direct exposure to light. Avoid freeze/thaw cycles.

Protease

Substrate