The Fluorogenic DPP8 Assay Kit is designed to measure DPP8 activity using purified DPP8 for screening and profiling applications. The key to the Fluorogenic DPP8 Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP8 reactions. The fluorometric substrate is incubated with a sample containing DPP8 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.

Dipeptidylpeptidase 8, MSTP141, DPP

Supplied in a convenient 96-well format, with purified DPP8 enzyme, DPP substrate, and DPP assay buffer for 100 enzyme reactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt

See assay kit data sheet for detailed protocol.

At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

1. Lee, H.J. et al., J. Biol. Chem. 281 (50), 38653-38662 (2006).
2. Yu, D.M. et al., FEBS J. 273 (11), 2447-2460 (2006).

Application Reference(s):
1. Screening of a natural compound library identifies emodin, a natural compound from Rheum palmatum Linn that inhibits DPP4 (2017)
2. Scaffold-based design of xanthine as highly potent inhibitors of DPP-IV for improving glucose homeostasis in DIO mice (2015)

The AMC standard is included so the researcher can quatitatively determine the specific activity of the enzyme using the AMC standard as a measure of how much AMC substrate was cleaved to release free AMC.

Avoid freeze/thaw cycles

Protease

Assay Kit