The Fluorogenic DPP3 Assay Kit is designed to measure DPP3 activity using purified DPP3 for screening or profiling applications. The key to the Fluorogenic DPP3 Assay Kit is the specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP3 reactions; no time-consuming washing steps are required. The fluorometric substrate is incubated with a sample containing DPP3 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.

Dipeptidylpeptidase 3, DPP

Supplied in a convenient 96-well format, with purified DPP3 enzyme, DPP substrate, and DPP assay buffer for 100 enzyme reactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt

See assay kit data sheet for detailed protocol.

At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

1. Gevaert,K. et al., Proteomics 5 (14), 3589- 3599 (2005).
2. Abramic,M. et al., Int. J. Biochem. Cell Biol. 36 (3), 434-446 (2004).

Application Reference(s):
1. Neuropeptide Kyotorphin Impacts on Lipopolysaccharide-Induced Glucocorticoid-Mediated Inflammatory Response. A Molecular Link to Nociception, Neuroprotection, and Anti-Inflammatory Action (2017)

The AMC standard is included so the researcher can quatitatively determine the specific activity of the enzyme using the AMC standard as a measure of how much AMC substrate was cleaved to release free AMC.

Avoid freeze/thaw cycles

Protease

Assay Kit