The TNKS1 Histone Ribosylation Colorimetric Assay Kit is designed to measure Tankyrase 1 (TNKS1) activity for screening and profiling applications. TNKS1 catalyzes the NAD-dependent addition of poly(ADP-ribose) to the substrate proteins. The key to the TNKS1 Histone Ribosylation Assay is the biotinylated NAD+ substrate. With this kit, only three simple steps are required for TNKS1 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated NAD+substrate is incubated with an assay buffer that contains the TNKS1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.

TNKS1, poly(ADP-ribose)polymerase 5a, poly(ADP-ribosyl)transferase 5a, PARP5A, TRF1-interacting ankyrin-related ADP-ribose polymerase 1, TANK1, tanykyrase-1

The TNKS1 assay kit comes in a convenient 96-well format, with purified TNKS1 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.

See assay kit data sheet for detailed protocol

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

Application Reference(s):
1. Small Molecules Inspired by the Natural Product Withanolides as Potent Inhibitors of Wnt Signaling (2017)

Avoid freeze/thaw cycles.

PARP

Assay Kits