Human PARP14 (GenBank Accession No. NM_017554), a.a. 1470-1801(end) with N-terminal His-GST-tag, MW = 66.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q460N5

poly (ADP-ribose) polymerase 14, poly(ADP-ribosyl)transferase 14, PARP14

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.

Enzyme reaction is conducted using BPS's PARP14 Assay Kit (Catalog #80568) at room temperature for 1 hour.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 188 mM imidazole, 0.04% Tween-20, 3 mM DTT, 20% glycerol.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Barbarulo, A., et al., Oncogene. 10 (1038), 448 (2012).
2. Cho, SH., et al., Proc Natl Acad Sci U S A. 108(38),15972-7 (2011).

Product Specific Reference(s):
1. Wang, P., et al., Tetrahedron. 70(35), 5666–5673 (2014).

Avoid freeze/thaw cycles.

PARP

Recombinant Protein