Human PDE10A2 (GenBank Accession No. NM_006661), full length with N-terminal GST-tag, MW= 116 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q9Y233

PDE10A2, phosphodiesterase 10A2

≥ 1600 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3' , 5' -cAMP to 5' -AMP per minute at 37°C.

10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 μM cAMP, and serial dilutions of PDE3A at 37ºC for 20 min. Quantified by 5 nucleotidase cleaving the 5-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween, 3mM DTT, and 20% Glycerol.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Gross-Langenhoff,M., et al., J. Biol. Chem. 281 (5), 2841-2846 (2006).
2. Frame,M., et al., Cell. Signal. 13 (10), 735- 741 (2001).

Avoid freeze/thaw cycles.

PDE

Recombinant Protein