Human PDE10A1 (GenBank Accession No. NM_001130690), a.a. 2-789(end) with N-terminal GST-tag, MW=116 kDa, expressed in Sf9 cells via a Baculovirus expression system.

Q9Y233

PDE10A1, phosphodiesterase 10A1

≥ 3200 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3' , 5' -cAMP to 5' -AMP per minute at 37°C.

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and 0.25-4 ng/µl PDE10A1 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 20 mM glutathione, 3 mM DTT and 10% glycerol.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Gross-Langenhoff,M., et al., J. Biol. Chem. 281 (5), 2841-2846 (2006).
2. Frame,M., et al., Cell. Signal. 13 (10), 735- 741 (2001).

Product Specific Reference(s):
1. Demirbas, D., et al., PLoS One. 8(8), (2013).
2. Wang, Z., et al., J Sex Med. 10(11), 2790-7 (2013).  

Application References:
1. Utility of Adenosine Monophosphate Detection System for Monitoring the Activities of Diverse Enzyme Reactions (2017)
2. Identification and characterization of a potent and biologically-active PDE4/7 inhibitor via fission yeast-based assays (2017)
3. Suppression of β-catenin/TCF transcriptional activity and colon tumor cell growth by dual inhibition of PDE5 and 10 (2015)

Avoid freeze/thaw cycles.

PDE

Recombinant Protein