Human PDE7A1 GenBank Accession No. NM_002603), a.a. 122-end with N-terminal GST-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q13946-2

PDE7A, phosphodiesterase 7A

≥ 180 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Assayed using BPS's PDE7A kit, Cat. # 60370. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE7A at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM Glutathione, 0.04% Tween-20, 3 mM DTT, and 20% glycerol.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Wang, H., et al., J. Biol. Chem. 280(35):30949-30955 (2005).
2. Torras-Llort, M. and Azorin, F., Biochem. J. 373(PT 3):835-843 (2003).

Application Reference(s):
1. Utility of Adenosine Monophosphate Detection System for Monitoring the Activities of Diverse Enzyme Reactions (2017)
2. Novel N-hydroxybenzamides incorporating 2-oxoindoline with unexpected potent histone deacetylase inhibitory effects and antitumor cytotoxicity. (2017)
3. Design and Synthesis of Novel and Selective Phosphodiesterase 2 (PDE2a) Inhibitors for the Treatment of Memory Disorders (2017)
4. Discovery of Selective Phosphodiesterase 1 Inhibitors with Memory Enhancing Properties (2017)
5. Genetic deletion and pharmacological inhibition of phosphodiesterase 10A protects mice from diet-induced obesity and insulin resistance (2014)
6. PDE7A1 hydrolyzes cCMP (2014)
7. The novel phosphodiesterase 10A inhibitor THPP-1 has antipsychotic-like effects in rat and improves cognition in rat and rhesus monkey (2013)

Avoid freeze/thaw cycles.

PDE

Recombinant Protein