Human PDE4D7 (GenBank Accession No. NM_001165899), a.a. 2-748(end) with N-terminal GST-tag, MW=111 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q08499

PDE4D7, phosphodiesterase 4D7

≥ 10,000 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4D7 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 20 mM Glutathione and 10% glycerol.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Wang, D., et al., (2003). Cell Signal. 15(9):883-91.
2. Houslay, M.D. (2005). Mol Pharmacol. (2005). 68(3):563-7

Avoid freeze/thaw cycles.

PDE

Recombinant Protein