Human PDE4B2 (GenBank Accession No. NM_001037339), full length, with N-terminal GST-tag and C-terminal His-tag, MW=91.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q07343

PDE4B2, phosphodiesterase 4B2

≥ 12,300 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Assayed using BPS's PDE4B2 Assay Kit, Cat. # 60343. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4B2 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween 20, and 20% glycerol.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Arp, J., et al., Mol Cell Biol. 2003 Nov; 23(22):8042-57.
2. Wang, P., et al., Mol Pharmacol. 1999 Jul; 56(1):170-4.

Application Reference(s):
1. Utility of Adenosine Monophosphate Detection System for Monitoring the Activities of Diverse Enzyme Reactions (2017)
2. Evaluation of the therapeutic index of a novel phosphodiesterase 4B-selective inhibitor over phosphodiesterase 4D in mice (2013)

Avoid freeze/thaw cycles.

PDE

Recombinant Protein