Human PDE4A4B, also known as PDE4A isoform 1 (GenBank Accession No. NM_001111307), a.a. (2-end) with N-terminal GST-tag, MW= 124 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

P27815

PDE4A isoform 1, PDE4A4B

≥ 11,610 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Assayed using BPS's PDE4A Assay Kit, Cat. # 60340. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4A4B at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 3 mM DTT and 20% glycerol

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Sullivan,M., et al., Journal Biochem. J. 333:693-703 (1998).
2. Huston E, et al., J Cell Sci. 2006 119:3799-810. (2006).

Avoid freeze/thaw cycles.

PDE

Recombinant Protein