Human Histone 3 (GenBank Accession No. NM_003532), a.a. 2-58 with N-terminal GST-tag, MW = 32 kDa, expressed in an E. coli expression system.

P68431

Histone 3.1, HIST1H3E

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT.

Aqueous buffer solution

Useful as a substrate for methyltransferases or acetyltransferases and for screening small molecular inhibitors of histone methyltransferases for drug discovery.

1. Loyola, A. et al., Mol. Cell 24 (2), 309-316 (2006).
2. Groth, A. et al., Mol. Cell 17 (2), 301-311 (2005).

Application Reference(s):
Differential mode of antimicrobial actions of arginine-rich and lysine-rich histones against Gram-positive Staphylococcus aureus (2013)

Avoid freeze/thaw cycles.

Methyltransferase

Substrate