Human VPS34 also known as vacuolar protein sorting 34, PI3-Kinase Type 3, and Phosphatidylinositol 3-Kinase Catalytic Subunit Type 3, GenBank Accession No. NM_002647, a.a. 2-887(end) with N-terminal FLAG-tag, expressed in Sf9 insect cells via a baculovirus expression system. MW=103 kDa.

Q8NEB9 P28221

VPS34, vacuolar protein sorting 34

120 pmol/min/μg.

Kinase buffer containing 2 mM McCl2 at 30°C for 20 min. Use BPS 4PI:PS substrate (100 μM) with 100 μM ATP. The amount of ATP transferred was calculated using ADP-Glo reagent.

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/mL FLAG peptide, 20% glycerol, 3 mM DTT

Aqueous buffer solution

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Yan, Y., et al. (2009). Biochem J. 417(3):747-55.
2. Backer, J.M. (2008). Biochem J. 410(1):1-17.
3. Juhász, G., et al. (2008). J Cell Biol. 181(4):655-66.

Application Reference(s):
1. Alteration of autophagy-related proteins in peripheral blood mononuclear cells of patients with Parkinson's disease (2017)

Avoid freeze/thaw cycles

Kinase/Lipids

Recombinant Protein