Recombinant full-length human SYK also known as Spleen Associated Tyrosine Kinase and Tyrosine-Protein Kinase SYK, GenBank Accession No. NM_003177, a.a. full length with N-terminal GST-tag, expressed in a Sf9 expression system. MW = 100 kDa.

P43405

SYK, Spleen Tyrosine Kinase SYK

108 pmol/min/µg

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.2 mg/ml Poly(Glu:Tyr) substrate and 20 uM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

45 mM Tris-HCl, pH 8, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione,3 mM DTT and 10% glycerol.

Aqueous buffer solution

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Takano, T. et al. Antioxid Redox Signal. 2002 Jun,4(3):533-41.
2. Navara, C S. Curr Pharm Des. 2004,10(15):1739-44.

Application Reference(s):
1. Reconstruction and signal propagation analysis of the Syk signaling network in breast cancer cells (2017)

Avoid freeze/thaw cycles

Kinase/Cytoplasmic Tyr

Recombinant Protein