Human Sphingosine kinase 1 or SphK1 (GenBank Accession No. AF200328), full length with N-terminal His-tag, MW=46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q9NYA1-1

SphK1, sphingosine kinase 1, SPK1

3,300 pmole/min/μg

Assay was done in Kinase buffer containing (40 mM Tris, pH 7.4, 20 mM MgCl2+, 0.1 mg/ml BSA) containing 20 μM Sphingosine and 20 μM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega) .

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 225 mM imidazole, and 3 mM DTT.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Delon, C., et al., J. Biol. Chem 279 (43) 44763-44774 (2004).
2. Taha, TA., et al., FASEB J. 20(3):482-4 (2005).

Application Reference:
Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation (2014)

Avoid freeze/thaw cycles

Kinase/Lipids

Recombinant Protein