Human p120γ (gamma) (GenBank Accession No. NM_002649), full length with N-terminal His-tag, MW=130 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

P48736

PI3CG, 3-kinase catalytic subunit gamma isoform; p120-PI3K; PI3K-gamma, p120γ

1200 pmol/min/μg.

A 25 μl kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.4), 20 mM MgCl₂, 100 uM ATP, 200 μM PI:PS substrate (BPS catalog #40560) and 0-200 ng p120γ enzyme for 15 minutes at 30°C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform (Promega Corporation, Madison, WI).

25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 3 mM DTT, and 50% glycerol

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Webb, LM., et al., J Immunol. 175(5):2783-7 (2005).
2. Voigt, P., et al., J Biol Chem. 280(6):5121-7(2005).

Application Reference:
Discovery of a potent and isoform-selective targeted covalent inhibitor of the lipid kinase PI3Kα (2013)

Avoid freeze/thaw cycles

Kinase/Lipids

Recombinant Protein