Human Insulin-like Growth Factor-1 Receptor (GenBank Accession No. NP_000875), a.a. 930-1337 containing the 46 kDa cytoplasmic domain of the beta-subunit of IGFR1 and an N-terminal GST-tag, MW=72.3 kDa, expressed in Sf9 cells via a baculovirus expression system.

IGF1R, Insulin-like Growth Factor-1 Receptor, CD221, JTK13, IGFIR

~100 nmol/min/mg

Assay was performed at 25°C using poly(Glu:Tyr)4:1 substrate. Reaction conditions are 50 mM Tris, pH 7.5, 5 mM MgCl₂, 5 mM MnCl₂, 1 mM DTT, 250 μM ATP. The kinase concentration was 0.5-1 μM and poly-L-lysine was added in equimolar amounts to the kinase.

50 mM Tris, pH 7.5 and 1 mM DTT.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Baer, K., et al., Biochemistry. 2001 Nov 27;40(47):14268-78.
2. Tennagels, N., et al., Biochem. Biophys. Res. Com. 1999 260: 724-8.

Application Reference:
A highly selective dual insulin receptor (IR)/insulin-like growth factor 1 receptor (IGF-1R) inhibitor derived from an extracellular signal-regulated kinase (ERK) inhibitor (2014)

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein