Recombinant human AKT1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal His-tag, MW=57 kDa, was expressed in Sf9 insect cells via a baculovirus expression system. The purified AKT1 is activated in vitro using PDK1 enzyme.

AKT1, PKBα, PKBalpha, RACalpha, AKT

12.32 pmol/min/μg

Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 6, Invitrogen) at room temperature for 1 hour.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.

Purified by affinity chromatography

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Kuijl, C., et al., Nature. 2007 Nov 29; 450(7170):725-30.
2. Vogiatzi, P, and Giordano, A., Cancer Biol Ther. 2007 Aug 3; 6(10).

Product Specific Reference(s): 
1. Koeberle, A., et al., Proc Natl Acad Sci U S A. 2013 Feb 12; 110(7):2546-51.

Avoid freeze/thaw cycles

Kinase/Serine-Threonine

Recombinant Protein