Human Abelson murine leukemia viral oncogene homolog 1 (Abl-1) (GenBank Accession No. NM_007313), a.a. 248-531, with N-terminal His-tag, MW = 33 kDa, expressed in a Baculovirus-infected Sf9 cell expression system

P00519

ABL1, ABL-1, Abelson murine leukemia viral oncogene homolog 1, JTK7

2,700 pmoles/min/μg.

Assay was performed in Kinase buffer containing 0.2 mM DTT using Abltide substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 30 min. Amount of ATP transferred was calculated using Kinase-Glo^® reagent (Promega).

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 200 mM imidazole, and 20% glycerol.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Cao, C., et al. 2003. J. Biol. Chem. 278(15):12961–7.
2. Yano, H., et al. 2000. J. Neurosci. Res. 59(3):356–364.

Application Reference:
LASP1 is a novel BCR-ABL substrate and a phosphorylation-dependent binding partner of CRKL in chronic myeloid leukemia (2014)

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein