The HSP90α Assay Kit is designed for identification of HSP90α inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin for binding to purified recombinant HSP90α. The key to the HSP90α Assay Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required for HSP90α reactions. The fluorescently labeled geldanamycin is incubated with a sample containing HSP90α enzyme to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.

P07900

HSP90a, Heat Shock Protein 90a, HSP90alpha, HSP90α

The HSP90α inhibitor screening assay kit comes in a convenient 96-well format, with purified HSP90α enzyme, fluorescently labeled geldanamycin and HSP90α assay buffer for 96 enzyme reactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt

See data sheet for detailed protocol.

Stable for at least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

1. Kim, J., et al. J Biomol Screening 2004, 9(5): 375-381.
2. Howes, R., et al. Anal Biochem 2006, 350:202-213.

Application Reference(s):
1. Rational Design of Selective Adenine-Based Scaffolds for Inactivation of Bacterial Histidine Kinases (2017)
2. The quinone methide aurin is a heat shock response inducer that causes proteotoxic stress and Noxa-dependent apoptosis in malignant melanoma cells (2015)
3. Herbimycins D-F, ansamycin analogues from Streptomyces sp. RM-7-15 (2013)

Avoid freeze/thaw cycles

Heat Shock Proteins

Assay Kit