Variant 2 Human LSD1 [also known as KDM1A (lysine (K)-specific demethylase 1A) or AOF2, amine oxidase (flavin containing) domain 2)] GenBank Accession No. NM_015013, a.a. 158-end with N-terminal GST-tag, MW=103 kDa, expressed in an E. coli expression system.

O60341

KDM1A, AOF2, lysine (K)-specific demethylase 1A, amine oxidase (flavin containing) domain 2, LSD

≥0.50 pmole/min/μg

10 μl reaction mix containing assay buffer with 50 mM Tris HCl pH 8.8, 50 mM NaCl, 1mM DTT, 0.01% Tween-20, histone H3 peptide substrate, and LSD1 (6.7 – 40 ng) add to the wells. Incubate for 1 hr. Add antibody against unmethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

100 mm Na2HPO4 /NaH2PO4, pH 7.4

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Lan,F. et al., Nature 448, 7154, 718-722, 2007.
2. Forneris,F. et al., J. Biol. Chem. 282, 28, 20070-20074, 2007.

Application Reference(s):
1. Design, synthesis and biological activity of 3-oxoamino-benzenesulfonamides as selective and reversible LSD1 inhibitors (2017)
2. A scintillation proximity assay for histone demethylases (2014)
3. Crystal structure of the histone lysine specific demethylase LSD1 complexed with tetrahydrofolate (2014)

LSD1,GST-tag (BPS Cat. #50204) is optimized for use with BPS Bioscience's LSD1 Fluorescent Assay Kits including BPS Cat #50106 and BPS Cat #50107. 

Avoid freeze/thaw cycles.

Demethylase

Recombinant Protein