Human JMJDE , also known as KDM4DL (GenBank Accession No. NM_001161630), a.a. 2-337 with N-terminal GST-tag, MW=65 kDa, expressed in Sf9 cells using a Baculovirus expression system.

B2RXH2

KDM4DL, JMJDE, KDM4D-Like

0.14 pmole/min/μg

10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2E (100 – 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Fodor, B.D., et al., (2006). Genes Dev. 20(12): 1557–1562.
2. Beyer, S., et al., (2008). J. Biol. Chem. 283(52):36542-52.

Application Reference(s):
A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

Avoid freeze/thaw cycles.

Demethylase

Recombinant Protein