Human Sirtuin 1 , GenBank Accession No. NM_012238, a.a. 193-741 with N-terminal GST-tag, MW= 88 kDa, expressed in an E. coli expression system.

Q96EB6

Sirtuin1, SIRT1, Sir2, Sir2a

≥20 pmol/min /µg

25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 1 (Catalog #50032), 500 µM NAD+ and Sirtuin 1 (12.5 - 800 ng). Incubation condition: 30 min at 37°C followed by treatment with 50 µl of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Kolthur-Seetharam, U. et al (2006) Cell Cycle 5, 873-877.
2. Solomon, J.M. et al (2006) Mol. Cell. Biol. 26, 28-38.

Application Reference(s):
1. SIRT4 Is a Lysine Deacylase that Controls Leucine Metabolism and Insulin Secretion (2017)
2. Lysine Deacetylases Exhibit Distinct Changes in Activity Profiles Due to Fluorophore Conjugation of Substrates (2017)
3. Development of High-Throughput Assays to Study Methylases, Demethylases and Deacetylases (2011)

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).

Deacetylase

Recombinant Protein