The Fluorogenic HDAC3 Assay Kit is a complete assay system designed to measure histone deacetylase 3 (HDAC3) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC3 activity measurements. In addition, the kit includes purified HDAC3 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC3 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

O15379

histone deacetylase, HDAC3

DMSO >1%, strong acids or bases, ionic detergents, high salt

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors of HDAC3 for drug discovery and HTS applications.

1. Jia, H., et al., Neurobiology of Disease. 2012 May; 46(2): 351-361
2. Bradner, J.E., et al., Nat Chem Biol. 2010 Mar; 6(3): 238-243.

Application Reference(s):
1. HDAC3 inhibition ameliorates spinal cord injury by immunomodulation (2017)
2. Preclinical anti-arthritic study and pharmacokinetic properties of a potent histone deacetylase inhibitor MPT0G009 (2014)

Avoid freeze/thaw cycles.

Deacetylase

Assay Kit