The Fluorogenic HDAC Assay Kit is a complete assay system designed to measure histone deacetylase (HDAC) class 1 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC activity measurements. In addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

histone deacetylase, HDAC

DMSO >1%, strong acids or bases, ionic detergents, high salt

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. This kit is suitable for class 1 and class 2b HDACs (HDACs 1, 2, 3, 6, 10). For class 2a and class 4 HDACs (HDACs 4, 5, 7, 9, 11), we reco

1. A. Ito et al., 2001, EMBO J. 20 1331.
2. N.A. Barlev et al., 2001, Mol. Cell 8 1243.
3. A. Ito et al., 2002, EMBO J. 21 6236.

Application Reference(s):
1. Discovery of a small molecule agonist of phosphatidylinositol 3-kinase p110α that reactivates latent HIV-1 (2014)
2. Hydroxamic acid-based histone deacetylase (HDAC) inhibitors can mediate neuroprotection independent of HDAC inhibition (2014)
3. Mitogen-Activated Protein Kinase Phosphatase 3 (MKP-3)-Deficient Mice Are Resistant to Diet-Induced Obesity (2014)

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
0.1% solution (1 mg/ml) of bovine serum albumin (BSA) in water
Fluorimeter capable of excitation at 350-380 nm and detection at 440-460 nm
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Avoid freeze/thaw cycles.

Deacetylase

Assay Kit