Human HDAC5 , GenBank Accession No. NM_005474, full length with N-terminal GST-tag, MW= 150 kDa, expressed in baculovirus expression system.

Q9UQL6

histone deacetylase 5, HDAC-5

One U =1 pmol of acetyl group removed/min/µg of enzyme

25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 µM BPS substrate(Catalog number 50040), and 0.8-50 ng/µl HDAC5. Incubation condition: 30 min at 37°C followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Spiegelberg, B.D. and Hamm, H.E. (2005) J. Biol. Chem. 280, 41769-41776
2. Waltregny et al., (2004) J. Neural Transm. 48, 273-290.

Application References:
1. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014)
2. A selective HDAC 1/2 inhibitor modulates chromatin and gene expression in brain and alters mouse behavior in two mood-related tests (2013)
3. Crebinostat: a novel cognitive enhancer that inhibits histone deacetylase activity and modulates chromatin-mediated neuroplasticity (2013)
4. Total synthesis and full histone deacetylase inhibitory profiling of Azumamides A-E as well as β²- epi-Azumamide E and β³-epi-Azumamide E.z

Avoid freeze/thaw cycles.

Deacetylase

Recombinant Protein